Array Replacement Case Study

For decades, methylation arrays have been a staple in epigenetic research due to their accessibility and well-established workflows. However, their reliance on predefined loci, bias toward pG islands, and dependence on bisulfite conversion introduce errors and limit scope. These limitations have driven the need for holistic, unbiased approaches that offer higher sensitivity and deeper insights, particularly in clinical epigenetics Renew Biotechnologies’ (Renew) Direct Whole Methylome Sequencing (dWMS) addresses this need by leveraging Oxford Nanopore Technologies' native long-read sequencing platform. This solution delivers robust genome-wide methylation data with extensive CpG coverage, eliminating the biases of bisulfite-based methods. Enhanced by the userfriendly ModSeqR toolkit, dWMS simplifies analysis and empowers researchers to explore clinical applications with precision and efficiency. Because dWMS uses the ONT platform, nanopore sequencing will be highlighted in this case study.

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