Products
Products
Explore our advanced sequencing products for turning complex molecular signals into high-confidence readouts across clinical testing, RNA quality control, and translational methylation programs.
NeuroLens®
Neuron Cell-Death Test
RNA Clarity™
RNA Vaccine QC
MethylMap™
Native-Read Methylation Sequencing
Older woman discussing a clinical diagnosis with a female doctor who is reviewing a tablet in a medical office.
Services
Services
End-to-end sequencing services supporting genomic, epigenomic, and transcriptomic analysis, from experimental design and assay development to bioinformatics, interpretation, and data delivery.
Applications
Applications
Explore how our products and services help solve difficult biological and genomic problems, from liquid biopsy signals to complex regions and emerging biomarker development.
Hard-to-Sequence Genes & Complex Regions
Liquid Biopsy cfDNA Analysis
Custom Multiomic Panel Development
Company
Company
Building the future of precision diagnostics through sequencing innovation, scientific rigor, and collaborative partnerships.
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Team
Resources
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  • Applications
  • Applications
    Telomeres, the protective caps at the end of our chromosomes, sit at the intersection of aging, cancer, and neurodegeneration research.
    Telomeres, the protective caps at the end of our chromosomes, sit at the intersection of aging.
    Research & Translation
    Biopharma Research
    Clinical Development
  • Company
  • Company
    Building the future of precision diagnostics through sequencing innovation, scientific rigor, and collaborative partnerships.
    About Us
    Team
Oxford Nanopore Technologies Service

Whole-Genome Sequencing

  • A native long-read whole-genome sequencing service for genome assembly, variant discovery, and multiomic characterization.
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Overview

Whole-Genome Sequencing is a standard Oxford Nanopore Technologies (ONT) service for native long-read sequencing across the genome. The workflow supports genome-wide analysis of sequence variation, structural variation, and methylation without bisulfite conversion or PCR amplification.
  • Long-read resolution across complex genomic regions
  • Native 5mC, 5hmC, and 6mA methylation detection
  • Integrated genomic and epigenetic analysis in one workflow
  • Flexible coverage options based on study goals and sample type
Genomic Variation Analysis
SNV, indel, and structural variant detection
Haplotype and phased genome analysis
Native Epigenomic Profiling
5mC, 5hmC, and 6mA methylation detection
Integrated genomic and epigenetic analysis
Research & Translational Applications
De novo genome assembly and annotation
Biomarker discovery and genome characterization
Technology
Workflow
Performance
Data Outputs

Technology and Compatibility

Whole-Genome Sequencing applies native ONT long-read sequencing to genomic DNA from diverse organisms and sample types, enabling integrated genomic and epigenomic analysis across a wide range of species.

  • Compatible with
  • DNA from human, animal, plant, microbial, and non-model organisms
  • Fresh, frozen, cultured, and extracted DNA sample types
  • Research, translational, and genome characterization workflows
  • Component
    Technology
  • Sequencing Chemistry
    Native Oxford Nanopore DNA Sequencing
  • Preparation
    Standard ONT-compatible DNA preparation
  • Sequencing Platform
    Oxford Nanopore PromethION

Workflow

Whole-Genome Sequencing uses ONT’s native long-read DNA sequencing workflow, with single-plex and multiplex options. Renew provides full-service support from gDNA extraction through library preparation, sequencing, analysis, and data delivery.
Diagram of high molecular weight gDNA steps: fragmentation, end-prep, barcode ligation, adapter ligation, loading.
Figure 1. Oxford Nanopore Technologies Whole-Genome Sequencing library preparation workflow (SQK-LSK114 or SQK-NBD114.96).
  • 1. gDNA Extraction
    High-quality genomic DNA is isolated from samples
  • 2. Library Preparation
    DNA is prepared using ONT’s SQK-LSK114 or SQK-NBD114.96
  • 3. Long-Read Sequencing
    Native DNA sequencing is performed on the PromethION platform.
  • 4. Data Analysis
    Sequencing data are processed through automated analysis pipelines.
  • 5. Data Delivery
    Results are delivered through the customer portal or AWS S3.

Sequencing Performance

Coverage and yield vary by genome size, input quality, multiplexing strategy, and study goals. Representative performance metrics are shown below.
Download Spec Sheet (Human)
Download Spec Sheet (Non-Human)
Table of genome size, average read length, and coverage for human, mouse, zebrafish, thale cress, and mung bean.
  • Metric
    Typical Performance
  • Samples per Flow Cell
    Up to 96
  • Average Coverage
    Dependent on genome size and desired depth
  • Average Read Length
    ~9 kb N50
  • CpG Coverage
    > 95% CpG coverage
  • Available Epigenetic Signals
    5mC, 5hmC, 6mA, non-CpG methylation

Bioinformatics and Data Outputs

Data outputs are designed to optimize data storage, transfer, and analysis. Methylation data is provided in a unique CH3 file format which reduces data size by up to 95%. This memory-efficient format stores data in an on-disk database, enabling larger-than-memory tasks.
Renew developed the ModSeqR R package, which converts ONT methylation data into array-like formats compatible with tools such as minfi, DMRcate, and chAMP. This solution empowers researchers to efficiently leverage dWMS data within familiar frameworks, facilitating advanced epigenetic insights.
Get ModSeqR on GitHub
Download panel showing four files for specimen 250409 with names, sizes, dates, and download options.
  • Data Outputs
  • QC Report
    Sequencing metrics and run statistics
  • BAM
    Aligned reads
  • CH3
    Base modifications
  • POD5
    Raw sequencing data (available for an additional fee)
  • Data Delivery Options
  • Client Web Portal
    Manual download | Mac, Linux, Windows
  • AWS S3 Transfer
    Automated process | AWS to AWS
  • Command Line Script
    Manual download | Mac, Linux

Service Inclusions

Each project is supported by dedicated project management and cross-functional expertise across assay development, bioinformatics, and regulatory strategy. Renew provides proactive communication, transparent project tracking, and technical support from study design through data delivery.
Renew combines platform flexibility, nanopore expertise, and operational excellence to resolve areas
of the genome that standard approaches cannot.
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