cDNA Sequencing for Isoform Detection Case Study

Long-read cDNA sequencing enables isoform-level resolution of transcriptomic changes that are not accessible with short-read methods. In this study, Oxford Nanopore Technologies (ONT) long-read sequencing was applied to whole-blood samples to evaluate its ability to detect treatment-associated splice variants and characterize transcriptome-wide expression changes. Results demonstrate the utility of full-length transcript sequencing for identifying isoform-level biomarkers and supporting translational applications in therapeutic development.

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